Application
GFM1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GFM1 antibody detected the GFM1 protein (arrow).Application
GFM1 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the GFM1 antibody detected the GFM1 protein (arrow).Application
GFM1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFM1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | GFM1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EFG, EFG1, GFM |
|---|---|
| Alternative Names | GFM1 EFG EFG1 GFM Elongation factor G mitochondrial Elongation factor G 1 mitochondrial Elongation factor G1 |
| Molecular Weight(MW) | 83471 Da |
| Function | Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis |
| Tissue Specificity | This GFM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 727-755 amino acids from the C-terminal region of human GFM1. |
| Cellular Localization | Mitochondrion (By similarity). |
| Entrez Gene | 85476 |
|---|---|
| Nucleotide Accession | NP_079272.4 |
Application
GFM1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GFM1 antibody detected the GFM1 protein (arrow).
Application
GFM1 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the GFM1 antibody detected the GFM1 protein (arrow).
Application
GFM1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFM1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
