Application
GFI1B Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the GFI1B antibody detected the GFI1B protein (arrow).Application
GFI1B Antibody (C-term) western blot analysis in U266 cell line lysates (35ug/lane).This demonstrates the GFI1B antibody detected the GFI1B protein (arrow).| Product Name | GFI1B Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Zinc finger protein Gfi-1b Zinc finger protein Gfi-1b Zinc finger protein Gfi-1b Growth factor independent protein 1B Zinc finger protein Gfi-1b Potential regulator of CDKN1A translocated in CML |
|---|---|
| Molecular Weight(MW) | 37492 Da |
| Function | Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down- regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma- satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation |
| Tissue Specificity | Expressed in bone marrow and fetal liver, but also detectable in fetal spleen, fetal thymus, and testes Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Overexpressed in bone marrow of patients with erythroleukemia and megakaryocytic leukemia as well as in their corresponding leukemic cell lines, and markedly repressed in severe aplastic anemia (SAA) |
| Cellular Localization | Nucleus. |
| Entrez Gene | 8328 |
|---|
Application
GFI1B Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the GFI1B antibody detected the GFI1B protein (arrow).
Application
GFI1B Antibody (C-term) western blot analysis in U266 cell line lysates (35ug/lane).This demonstrates the GFI1B antibody detected the GFI1B protein (arrow).| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
