Application
Western blot analysis of GFPT1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). GFPT1 (arrow) was detected using the purified Pab.Application
GFPT1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFPT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
GFPT1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of GFPT1 Antibody (N-term)(Cat#AP8887a) with HepG2 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | GFPT1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GFAT, GFPT |
|---|---|
| Alternative Names | GFPT1 GFAT GFPT Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 D-fructose-6-phosphate amidotransferase 1 Glutamine:fructose 6 phosphate amidotransferase 1 Hexosephosphate aminotransferase 1 |
| Molecular Weight(MW) | 78806 Da |
| Function | Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins |
| Tissue Specificity | Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle |
| Entrez Gene | 2673 |
|---|
Application
Western blot analysis of GFPT1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). GFPT1 (arrow) was detected using the purified Pab.
Application
GFPT1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFPT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
GFPT1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of GFPT1 Antibody (N-term)(Cat#AP8887a) with HepG2 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
