Application
GIT1-S388 Antibody western blot analysis in Ramos cell line lysates (15ug/lane).This demonstrates the GIT1 antibody detected GIT1 protein (arrow).Application
GIT1-S388 Antibody western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the GIT1 antibody detected GIT1 protein (arrow).Application
GIT1-S388 Antibody flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GIT1-S388 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GIT1 ARF GTPase-activating protein GIT1 Cool-associated and tyrosine-phosphorylated protein 1 G protein-coupled receptor kinase-interactor 1 GRK-interacting protein 1 |
|---|---|
| Molecular Weight(MW) | 84341 Da |
| Function | GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine- phosphorylated paxillin in cytoplasmic complexes |
| Tissue Specificity | This GIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-403 amino acids from human GIT1. |
| Cellular Localization | Cytoplasm. Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin |
| Entrez Gene | 28964 |
|---|---|
| Nucleotide Accession | NP_054749.2 NP_001078923.1 |
Application
GIT1-S388 Antibody western blot analysis in Ramos cell line lysates (15ug/lane).This demonstrates the GIT1 antibody detected GIT1 protein (arrow).
Application
GIT1-S388 Antibody western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the GIT1 antibody detected GIT1 protein (arrow).
Application
GIT1-S388 Antibody flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
