Application
Western blot analysis of GIT1 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). GIT1 (arrow) was detected using the purified Pab.Application
Western blot analysis of GIT1 Antibody (C-term) in mouse brain tissue lysates (35ug/lane). GIT1 (arrow) was detected using the purified Pab.Application
GIT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GIT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
GIT1 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GIT1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GIT1 ARF GTPase-activating protein GIT1 Cool-associated and tyrosine-phosphorylated protein 1 G protein-coupled receptor kinase-interactor 1 GRK-interacting protein 1 |
|---|---|
| Molecular Weight(MW) | 84341 Da |
| Function | GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine- phosphorylated paxillin in cytoplasmic complexes |
| Tissue Specificity | This GIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 653~682 amino acids from the C-terminal region of human GIT1. |
| Cellular Localization | Cytoplasm. Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin |
| Entrez Gene | 28964 |
|---|
Application
Western blot analysis of GIT1 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). GIT1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of GIT1 Antibody (C-term) in mouse brain tissue lysates (35ug/lane). GIT1 (arrow) was detected using the purified Pab.
Application
GIT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GIT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
GIT1 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
