Application
Western blot analysis of hGJA7-H104.Connexin in MDA-MB435 cell line lysates (35ug/lane).GJA7 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | GJA7 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GJC1; GJA7; Gap junction gamma-1 protein; Connexin-45; Gap junction alpha-7 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GJA7 |
|---|---|
| Alternative Names | GJC1 GJA7 Gap junction gamma-1 protein Connexin-45 Gap junction alpha-7 protein |
| Molecular Weight(MW) | 45470 Da |
| Function | One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell |
| Tissue Specificity | This GJA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~127 amino acids from the N-terminal region of human GJA7. |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Cell junction, gap junction |
| Entrez Gene | 10052 |
|---|
Application
Western blot analysis of hGJA7-H104.Connexin in MDA-MB435 cell line lysates (35ug/lane).GJA7 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
