Application
Western blot analysis of GK (arrow) using rabbit polyclonal GK Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GK gene.Application
GK Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GK Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | GK Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GK; Glycerol kinase; ATP:glycerol 3-phosphotransferase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GK Glycerol kinase ATP:glycerol 3-phosphotransferase |
|---|---|
| Molecular Weight(MW) | 61245 Da |
| Function | Key enzyme in the regulation of glycerol uptake and metabolism |
| Tissue Specificity | Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver |
| Cellular Localization | Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Note=In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm |
| Entrez Gene | 2710 |
|---|---|
| Nucleotide Accession | NP_976325.1 NP_001121599.1 |
Application
Western blot analysis of GK (arrow) using rabbit polyclonal GK Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GK gene.
Application
GK Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GK Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
