Application
Western blot analysis of GLA antibody (N-term) in Hela cell line lysates (35ug/lane). GLA (arrow) was detected using the purified Pab.Application
GLA Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of GLA Antibody (N-term) with Hela cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
GLA Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GLA Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLA; Alpha-galactosidase A; Alpha-D-galactosidase A; Alpha-D-galactoside galactohydrolase; Melibiase; INN=Agalsidase; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GLA Alpha-galactosidase A Alpha-D-galactosidase A Alpha-D-galactoside galactohydrolase Melibiase INN=Agalsidase Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 48767 Da |
| Tissue Specificity | This GLA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90~120 amino acids from the N-terminal region of human GLA. |
| Cellular Localization | Lysosome. |
| Entrez Gene | 2717 |
|---|
Application
Western blot analysis of GLA antibody (N-term) in Hela cell line lysates (35ug/lane). GLA (arrow) was detected using the purified Pab.
Application
GLA Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of GLA Antibody (N-term) with Hela cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
GLA Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
