Application
(LEFT)Western blot analysis of GLD2 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(MIDDLE)Western blot analysis of GLD2 Antibody (N-term) in mouse heart tissue lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of GLD2 Antibody (N-term) in CHO cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.Application
GLD2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | GLD2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GLD2 |
|---|---|
| Alternative Names | PAPD4 GLD2 Poly(A) RNA polymerase GLD2 PAP-associated domain-containing protein 4 Terminal uridylyltransferase 2 |
| Molecular Weight(MW) | 56028 Da |
| Function | Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation |
| Tissue Specificity | Expressed in brain. Within brain, it is expressed in cerebellum, hippocampus and medulla |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity) |
| Entrez Gene | 167153 |
|---|
Application
(LEFT)Western blot analysis of GLD2 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(MIDDLE)Western blot analysis of GLD2 Antibody (N-term) in mouse heart tissue lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of GLD2 Antibody (N-term) in CHO cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.
Application
GLD2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
