Application
Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.Application
GLO1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GLO1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of GLO1 Antibody (Center)(Cat#AP12898c) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | GLO1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLO1; Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GLO1 Lactoylglutathione lyase Aldoketomutase Glyoxalase I Ketone-aldehyde mutase Methylglyoxalase S-D-lactoylglutathione methylglyoxal lyase |
|---|---|
| Molecular Weight(MW) | 20778 Da |
| Function | Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B |
| Tissue Specificity | This GLO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the Central region of human GLO1. |
| Entrez Gene | 2739 |
|---|---|
| Nucleotide Accession | NP_006699.2 |
Application
Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.
Application
GLO1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GLO1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of GLO1 Antibody (Center)(Cat#AP12898c) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
