Application
Western blot analysis of GLRX2 antibody (C-term T135) in mouse cerebellum tissue lysates (35ug/lane). GLRX2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain reacted with GLRX2 Antibody (C-term T135), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of hela cells using GLRX2 Antibody (C-term T135)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GLRX2 Antibody (C-term T135) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLRX2; GRX2; Glutaredoxin-2, mitochondrial |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GRX2 |
|---|---|
| Alternative Names | GLRX2 GRX2 Glutaredoxin-2 mitochondrial |
| Molecular Weight(MW) | 18052 Da |
| Function | Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release |
| Tissue Specificity | Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes |
| Cellular Localization | Isoform 1: Mitochondrion. |
| Entrez Gene | 51022 |
|---|
Application
Western blot analysis of GLRX2 antibody (C-term T135) in mouse cerebellum tissue lysates (35ug/lane). GLRX2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain reacted with GLRX2 Antibody (C-term T135), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of hela cells using GLRX2 Antibody (C-term T135)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
