Application
Western blot analysis of GOLPH3 antibody (N-term) in Hela cell line lysates (35ug/lane). GOLPH3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with GOLPH3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of hela cells using GOLPH3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GOLPH3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GOLPH3; GPP34; Golgi phosphoprotein 3; Coat protein GPP34; Mitochondrial DNA absence factor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GPP34 |
|---|---|
| Alternative Names | GOLPH3 GPP34 Golgi phosphoprotein 3 Coat protein GPP34 Mitochondrial DNA absence factor |
| Molecular Weight(MW) | 33811 Da |
| Function | Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene |
| Tissue Specificity | Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers |
| Cellular Localization | Cell membrane (By similarity). Endosome (By similarity). Cytoplasm. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion intermembrane space. Note=Phosphatidylinositol 4-phosphate (PtdIns4P) binding is essential for Golgi localization |
| Entrez Gene | 64083 |
|---|
Application
Western blot analysis of GOLPH3 antibody (N-term) in Hela cell line lysates (35ug/lane). GOLPH3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with GOLPH3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of hela cells using GOLPH3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
