Application
Western blot analysis of GPA33 Antibody (C-term) in K562, 293, MDA-MB231 cell line lysates (35ug/lane). GPA33 (arrow) was detected using the purified Pab.Application
GPA33 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPA33 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
GPA33 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GPA33 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPA33; Cell surface A33 antigen; Glycoprotein A33 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GPA33 Cell surface A33 antigen Glycoprotein A33 |
|---|---|
| Molecular Weight(MW) | 35632 Da |
| Function | May play a role in cell-cell recognition and signaling. |
| Tissue Specificity | Expressed in normal gastrointestinal epithelium and in 95% of colon cancers |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 10223 |
|---|
Application
Western blot analysis of GPA33 Antibody (C-term) in K562, 293, MDA-MB231 cell line lysates (35ug/lane). GPA33 (arrow) was detected using the purified Pab.
Application
GPA33 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPA33 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
GPA33 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
