Application
Western blot analysis of GPI Antibody (C-term) in mouse Neuro-2a cell lysates (35ug/lane).GPI (arrow) was detected using the purified Pab.Application
GPI Antibody (C-term) western blot analysis in A2058(lane 1),Y79(lane 2),Ramos(lane 3),A375(lane 4),K562(lane 5) and mouse Neuro-2a(lane 6) cell line lysates (35ug/lane).This demonstrates the GPI antibody detected the GPI protein (arrow).Application
GPI Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GPI Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPI; Glucose-6-phosphate isomerase; Autocrine motility factor; Neuroleukin; Phosphoglucose isomerase; Phosphohexose isomerase; Sperm antigen 36 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GPI Glucose-6-phosphate isomerase Autocrine motility factor Neuroleukin Phosphoglucose isomerase Phosphohexose isomerase Sperm antigen 36 |
|---|---|
| Molecular Weight(MW) | 63147 Da |
| Function | Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons |
| Tissue Specificity | This GPI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452~481 amino acids from the C-terminal region of human GPI. |
| Cellular Localization | Cytoplasm. Secreted. |
| Entrez Gene | 2821 |
|---|
Application
Western blot analysis of GPI Antibody (C-term) in mouse Neuro-2a cell lysates (35ug/lane).GPI (arrow) was detected using the purified Pab.
Application
GPI Antibody (C-term) western blot analysis in A2058(lane 1),Y79(lane 2),Ramos(lane 3),A375(lane 4),K562(lane 5) and mouse Neuro-2a(lane 6) cell line lysates (35ug/lane).This demonstrates the GPI antibody detected the GPI protein (arrow).
Application
GPI Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
