Application
GPX4 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the GPX4 antibody detected the GPX4 protein (arrow).Application
GPX4 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of GPX4 Antibody (Center) (Cat#AP10137c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | GPX4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPX4; Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; Glutathione peroxidase 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GPX4 Phospholipid hydroperoxide glutathione peroxidase mitochondrial Glutathione peroxidase 4 |
|---|---|
| Molecular Weight(MW) | 22175 Da |
| Function | Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity) |
| Tissue Specificity | Present primarily in testis. |
| Cellular Localization | Isoform Mitochondrial: Mitochondrion. |
| Entrez Gene | 2879 |
|---|---|
| Nucleotide Accession | NP_001034937.1 |
Application
GPX4 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the GPX4 antibody detected the GPX4 protein (arrow).
Application
GPX4 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of GPX4 Antibody (Center) (Cat#AP10137c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
