Application
Western blot analysis of GRB14 Antibody (N-term) in mouse liver tissue lysates (35ug/lane). GRB14 (arrow) was detected using the purified Pab.Application
GRB14 Antibody (N-term) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GRB14 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GRB14; Growth factor receptor-bound protein 14; GRB14 adapter protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GRB14 Growth factor receptor-bound protein 14 GRB14 adapter protein |
|---|---|
| Molecular Weight(MW) | 60988 Da |
| Function | Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR) Potent inhibitor of insulin-stimulated MAPK3 phosphorylation Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal |
| Tissue Specificity | Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle |
| Cellular Localization | Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note=Upon insulin stimulation, translocates to the plasma membrane |
| Entrez Gene | 2888 |
|---|
Application
Western blot analysis of GRB14 Antibody (N-term) in mouse liver tissue lysates (35ug/lane). GRB14 (arrow) was detected using the purified Pab.
Application
GRB14 Antibody (N-term) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
