Application
A. Western blot analysis on 293 cell lysate using anti-GRB2 (C-term) RabMAb (Cat. #AJ1331a), dilution 1:10,000.Application
B. Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti- GRB2 (C-term) RabMAb (Cat. #AJ1331a).Application
C. Immunofluorescent staining of HeLa cells using anti-GRB2 (C-term) RabMAb (Cat. #AJ1331a).| Product Name | GRB2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GRB2, ASH, Growth factor receptor-bound protein 2, Adapter protein GRB2;SH2/SH3 adapter GRB2;Protein Ash |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y237 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ASH |
|---|---|
| Alternative Names | GRB2 ASH Growth factor receptor-bound protein 2 Adapter protein GRB2 SH2/SH3 adapter GRB2 Protein Ash |
| Molecular Weight(MW) | 25206 Da |
| Function | Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway |
| Tissue Specificity | A synthetic peptide corresponding to residues in the C-terminus of human GRB2 was used as an immunogen. This antibody can also detect the splice isoform GRB3-3 of GRB2. |
| Cellular Localization | Nucleus. Cytoplasm. Endosome. Golgi apparatus (By similarity) |
| Entrez Gene | 2885 |
|---|
Application
A. Western blot analysis on 293 cell lysate using anti-GRB2 (C-term) RabMAb (Cat. #AJ1331a), dilution 1:10,000.
Application
B. Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti- GRB2 (C-term) RabMAb (Cat. #AJ1331a).
Application
C. Immunofluorescent staining of HeLa cells using anti-GRB2 (C-term) RabMAb (Cat. #AJ1331a).| Application Notes | WB:1:1000~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
