Application
Western blot analysis of GRB2 (arrow) using rabbit polyclonal GRB2 Antibody (pY209) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GRB2 gene.Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRB2 Antibody (Y209), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | G_2 Antibody (Y209) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GRB2; ASH; Growth factor receptor-bound protein 2; Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ASH |
|---|---|
| Alternative Names | GRB2 ASH Growth factor receptor-bound protein 2 Adapter protein GRB2 Protein Ash SH2/SH3 adapter GRB2 |
| Molecular Weight(MW) | 25206 Da |
| Function | Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway |
| Tissue Specificity | This GRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y209 of human GRB2. |
| Cellular Localization | Nucleus. Cytoplasm. Endosome. Golgi apparatus (By similarity) |
| Entrez Gene | 2885 |
|---|
Application
Western blot analysis of GRB2 (arrow) using rabbit polyclonal GRB2 Antibody (pY209) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GRB2 gene.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRB2 Antibody (Y209), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
