Application
Western blot analysis of anti-GRK6 Pab in HeLa cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2). GRK6 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
GRK6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GRK6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | GRK6 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GRK6; GPRK6; G protein-coupled receptor kinase 6; G protein-coupled receptor kinase GRK6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GPRK6 |
|---|---|
| Alternative Names | GRK6 GPRK6 G protein-coupled receptor kinase 6 G protein-coupled receptor kinase GRK6 |
| Molecular Weight(MW) | 65991 Da |
| Function | Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro) |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Membrane; Lipid-anchor. |
| Entrez Gene | 2870 |
|---|
Application
Western blot analysis of anti-GRK6 Pab in HeLa cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2). GRK6 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
GRK6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GRK6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
