Application
A. Western blot analysis on 293 cell lysates using anti-Phospho-GSK3a/b (pY279/pY216) RabMAb (Cat. #AJ1334d), 1:2000 dilution. Cells were either (A) untreated (B) treated with APApplication
B. Immunohistochemical analysis of parafffin-embedded human brain using anti-GSK3aGSK3b (pY279)/(pY216) RabMAb (Cat. #AJ1334d).| Product Name | GSK3 Antibody alpha/beta Phospho (pY279)/(pY216) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GSK3A, Glycogen synthase kinase-3 alpha |
| Modification | p-Y216 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR933Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GSK3A Glycogen synthase kinase-3 alpha |
|---|---|
| Molecular Weight(MW) | 50981 Da |
| Function | Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1 Requires primed phosphorylation of the majority of its substrates Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding tyrosine 279 in human GSK3a and tyrosine 216 in human GSKb was used as an immunogen. This antibody detects both GSKα phosphorylated on tyrosine 279 and GSK3β phosphorylyated on tyrosine 216. |
| Entrez Gene | 2931 |
|---|
Application
A. Western blot analysis on 293 cell lysates using anti-Phospho-GSK3a/b (pY279/pY216) RabMAb (Cat. #AJ1334d), 1:2000 dilution. Cells were either (A) untreated (B) treated with AP
Application
B. Immunohistochemical analysis of parafffin-embedded human brain using anti-GSK3aGSK3b (pY279)/(pY216) RabMAb (Cat. #AJ1334d).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
