Application
Western blot analysis of GSN antibody (N-term) (Cat.# AP7326a) in A375 cell line lysates (35ug/lane). GSN (arrow) was detected using the purified Pab.Application
GSN Antibody (N-term) (RB18721) IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
GSN Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GSN Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GSN; Gelsolin; AGEL; Actin-depolymerizing factor; Brevin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GSN Gelsolin AGEL Actin-depolymerizing factor Brevin |
|---|---|
| Molecular Weight(MW) | 85698 Da |
| Function | Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis |
| Tissue Specificity | Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells |
| Cellular Localization | Isoform 2: Cytoplasm, cytoskeleton. |
| Entrez Gene | 2934 |
|---|
Application
Western blot analysis of GSN antibody (N-term) (Cat.# AP7326a) in A375 cell line lysates (35ug/lane). GSN (arrow) was detected using the purified Pab.
Application
GSN Antibody (N-term) (RB18721) IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
GSN Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
