Application
GSPT1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSPT1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
GSPT1 Antibody (N-term) western blot analysis in U251,CEM cell line lysates (35ug/lane).This demonstrates the GSPT1 antibody detected the GSPT1 protein (arrow).Application
GSPT1 Antibody (N-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GSPT1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GSPT1; ERF3A; Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; G1 to S phase transition protein 1 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBFC |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ERF3A |
|---|---|
| Alternative Names | GSPT1 ERF3A Eukaryotic peptide chain release factor GTP-binding subunit ERF3A G1 to S phase transition protein 1 homolog |
| Molecular Weight(MW) | 55756 Da |
| Function | Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons |
| Tissue Specificity | This GSPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human GSPT1. |
| Entrez Gene | 2935 |
|---|---|
| Nucleotide Accession | NP_001123479.1 |
Application
GSPT1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSPT1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
GSPT1 Antibody (N-term) western blot analysis in U251,CEM cell line lysates (35ug/lane).This demonstrates the GSPT1 antibody detected the GSPT1 protein (arrow).
Application
GSPT1 Antibody (N-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
