Application
Western blot analysis of GZMM Antibody (Center) in K562 cell line lysates (35ug/lane). GZMM (arrow) was detected using the purified Pab.Application
GZMM Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GZMM Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
GZMM Antibody (Center) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GZMM Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GZMM; MET1; Granzyme M; Met-1 serine protease; Met-ase; Natural killer cell granular protease |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MET1 |
|---|---|
| Alternative Names | GZMM MET1 Granzyme M Met-1 serine protease Met-ase Natural killer cell granular protease |
| Molecular Weight(MW) | 27545 Da |
| Function | Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha- tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells |
| Tissue Specificity | Highly and constitutively expressed in activated natural killer (NK) cells |
| Cellular Localization | Secreted. Cytoplasmic granule. Note=Granules of large granular lymphocytes |
| Entrez Gene | 3004 |
|---|
Application
Western blot analysis of GZMM Antibody (Center) in K562 cell line lysates (35ug/lane). GZMM (arrow) was detected using the purified Pab.
Application
GZMM Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GZMM Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
GZMM Antibody (Center) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
