Application
Western blot analysis of GZMB Antibody (C-term) in HL-60 cell line lysates (35ug/lane). GZMB (arrow) was detected using the purified Pab.Application
GZMB Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GZMB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
GZMB Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of GZMB (arrow) using rabbit polyclonal GZMB Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.| Product Name | GZMB Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GZMB; CGL1; CSPB; CTLA1; GRB; Granzyme B; C11; CTLA-1; Cathepsin G-like 1; Cytotoxic T-lymphocyte proteinase 2; Fragmentin-2; Granzyme-2; Human lymphocyte protein; SECT; T-cell serine protease 1-3E |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CGL1 CSPB CTLA1 GRB |
|---|---|
| Alternative Names | GZMB CGL1 CSPB CTLA1 GRB Granzyme B C11 CTLA-1 Cathepsin G-like 1 Cytotoxic T-lymphocyte proteinase 2 Fragmentin-2 Granzyme-2 Human lymphocyte protein SECT T-cell serine protease 1-3E |
| Molecular Weight(MW) | 27716 Da |
| Function | This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis |
| Tissue Specificity | This GZMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182~211 amino acids from the C-terminal region of human GZMB. |
| Cellular Localization | Cytoplasmic granule. Note=Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells |
| Entrez Gene | 3002 |
|---|
Application
Western blot analysis of GZMB Antibody (C-term) in HL-60 cell line lysates (35ug/lane). GZMB (arrow) was detected using the purified Pab.
Application
GZMB Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GZMB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
GZMB Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of GZMB (arrow) using rabbit polyclonal GZMB Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
