Application
Western blot analysis of GZMH antibody (N-term) in Jurkat cell line lysates (35ug/lane). GZMH (arrow) was detected using the purified Pab.Application
GZMH Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GZMH Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
GZMH Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GZMH Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GZMH; CGL2; CTSGL2; Granzyme H; CCP-X; Cathepsin G-like 2; Cytotoxic T-lymphocyte proteinase; Cytotoxic serine protease C |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CGL2 CTSGL2 |
|---|---|
| Alternative Names | GZMH CGL2 CTSGL2 Granzyme H CCP-X Cathepsin G-like 2 Cytotoxic T-lymphocyte proteinase Cytotoxic serine protease C |
| Molecular Weight(MW) | 27315 Da |
| Function | Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses |
| Tissue Specificity | This GZMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12~42 amino acids from the N-terminal region of human GZMH. |
| Cellular Localization | Cytoplasmic granule. Note=Cytoplasmic granules of cytolytic T-lymphocytes |
| Entrez Gene | 2999 |
|---|
Application
Western blot analysis of GZMH antibody (N-term) in Jurkat cell line lysates (35ug/lane). GZMH (arrow) was detected using the purified Pab.
Application
GZMH Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GZMH Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
GZMH Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
