Application
Western blot analysis of H2AB2 Antibody (Center) in Jurkat cell line lysates (35ug/lane). H2AB2 (arrow) was detected using the purified Pab.Application
H2AB2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the H2AB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
H2AB2 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | H2AB2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H2AFB2; Histone H2A-Bbd type 2/3; H2A Barr body-deficient |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | H2AFB2 Histone H2A-Bbd type 2/3 H2A Barr body-deficient |
|---|---|
| Molecular Weight(MW) | 12713 Da |
| Function | Atypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase. They associate with spliceosome components and are required for mRNA splicing. May participate in spermatogenesis |
| Tissue Specificity | Present in mature sperm. |
| Cellular Localization | Nucleus. Chromosome. Note=Associated with the active X chromosome and with autosomes, while it is absent from the inactive X chromosome and excluded from Barr bodies |
| Entrez Gene | 83740 |
|---|
Application
Western blot analysis of H2AB2 Antibody (Center) in Jurkat cell line lysates (35ug/lane). H2AB2 (arrow) was detected using the purified Pab.
Application
H2AB2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the H2AB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
H2AB2 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
