Application
H2AFV Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (15ug/lane).This demonstrates the H2AFV antibody detected the H2AFV protein (arrow).Application
H2AFV Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the H2AFV Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
H2AFV Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | H2AFV Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H2AFV; H2AV; Histone H2A.V; H2A.F/Z |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H2AV |
|---|---|
| Alternative Names | H2AFV H2AV Histone H2A.V H2A.F/Z |
| Molecular Weight(MW) | 13509 Da |
| Function | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division (By similarity) |
| Tissue Specificity | This H2AFV antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H2AFV. |
| Cellular Localization | Nucleus (By similarity). Chromosome (By similarity) |
| Entrez Gene | 94239 |
|---|---|
| Nucleotide Accession | NP_036544.1 |
Application
H2AFV Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (15ug/lane).This demonstrates the H2AFV antibody detected the H2AFV protein (arrow).
Application
H2AFV Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the H2AFV Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
H2AFV Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
