Application
HADH antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HADH antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HADH Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
HADH Antibody (Center) western blot analysis in mouse spleen,bladder tissue and WiDr,MCF-7 cell line lysates (35ug/lane).This demonstrates the HADH antibody detected the HADH protein (arrow).| Product Name | HADH Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HADH; HAD; HADHSC; SCHAD; Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCFCWB |
| IHC:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HAD HADHSC SCHAD |
|---|---|
| Alternative Names | HADH HAD HADHSC SCHAD Hydroxyacyl-coenzyme A dehydrogenase mitochondrial Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase Short-chain 3-hydroxyacyl-CoA dehydrogenase |
| Molecular Weight(MW) | 34294 Da |
| Function | Plays an essential role in the mitochondrial beta- oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA |
| Tissue Specificity | Expressed in liver, kidney, pancreas, heart and skeletal muscle |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 3033 |
|---|---|
| Nucleotide Accession | NP_005318.2 |
Application
HADH antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HADH antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HADH Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
HADH Antibody (Center) western blot analysis in mouse spleen,bladder tissue and WiDr,MCF-7 cell line lysates (35ug/lane).This demonstrates the HADH antibody detected the HADH protein (arrow).| Application Notes | IHC:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
