Application
HARS Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the HARS antibody detected the HARS protein (arrow).Application
HARS Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HARS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HARS Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HARS Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HARS; HRS; Histidine--tRNA ligase, cytoplasmic; Histidyl-tRNA synthetase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HRS |
|---|---|
| Alternative Names | HARS HRS Histidine--tRNA ligase cytoplasmic Histidyl-tRNA synthetase |
| Molecular Weight(MW) | 57411 Da |
| Tissue Specificity | Brain, heart, liver and kidney. |
| Cellular Localization | Cytoplasm. |
Application
HARS Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the HARS antibody detected the HARS protein (arrow).
Application
HARS Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HARS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HARS Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
