Application
The anti-HDAC1 Pab is used in Western blot to detect HDAC1 in ZR-75-1 cell lysate.Application
The anti-HDAC1 Pab was used in Western Blot to detect HDAC1 in HEK293 cells.Knockdown of HDAC1 using siRNA against HDAC1 showed a significant decrease of HDAC1 protein using this anti-HDAC1 Pab in HEK293 cells.Application
Confocal immunofluorescent analysis of HDAC1 Antibody (C-term)(Cat#AP1101a) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Product Name | HDAC1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HDAC1; RPD3L1; Histone deacetylase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RPD3L1 |
|---|---|
| Alternative Names | HDAC1 RPD3L1 Histone deacetylase 1 |
| Molecular Weight(MW) | 55103 Da |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST- mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Component a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development |
| Tissue Specificity | Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain |
| Cellular Localization | Nucleus. |
| Entrez Gene | 3065 |
|---|
Application
The anti-HDAC1 Pab is used in Western blot to detect HDAC1 in ZR-75-1 cell lysate.
Application
The anti-HDAC1 Pab was used in Western Blot to detect HDAC1 in HEK293 cells.Knockdown of HDAC1 using siRNA against HDAC1 showed a significant decrease of HDAC1 protein using this anti-HDAC1 Pab in HEK293 cells.
Application
Confocal immunofluorescent analysis of HDAC1 Antibody (C-term)(Cat#AP1101a) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
