Application
Western blot analysis of anti-HDAC11 Pab in mouse brain tissue lysate. HDAC11 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Western blot analysis of HDAC11 (arrow) using HDAC11 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC11 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
HDAC11 Antibody (M1) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the HDAC11 antibody detected the HDAC11 protein (arrow).| Product Name | HDAC11 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HDAC11; Histone deacetylase 11 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HDAC11 Histone deacetylase 11 |
|---|---|
| Molecular Weight(MW) | 39183 Da |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes |
| Tissue Specificity | Weakly expressed in most tissues. Strongly expressed in brain, heart, skeletal muscle, kidney and testis |
| Cellular Localization | Nucleus. Note=Predominantly nuclear. |
Application
Western blot analysis of anti-HDAC11 Pab in mouse brain tissue lysate. HDAC11 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Western blot analysis of HDAC11 (arrow) using HDAC11 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC11 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
HDAC11 Antibody (M1) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the HDAC11 antibody detected the HDAC11 protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
