Application
The anti-HDAC3 Pab is used in Western blot to detect HDAC3 in mouse brain tissue lysate.Application
HDAC3 Antibody (M1) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the HDAC3 antibody detected the HDAC3 protein (arrow).Application
Western blot analysis of HDAC3 (arrow) using rabbit polyclonal HDAC3 Antibody (M1) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC3 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HDAC3 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | HDAC3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HDAC3; Histone deacetylase 3; RPD3-2; SMAP45 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HDAC3 Histone deacetylase 3 RPD3-2 SMAP45 |
|---|---|
| Molecular Weight(MW) | 48848 Da |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (By similarity) |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 8841 |
|---|
Application
The anti-HDAC3 Pab is used in Western blot to detect HDAC3 in mouse brain tissue lysate.
Application
HDAC3 Antibody (M1) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the HDAC3 antibody detected the HDAC3 protein (arrow).
Application
Western blot analysis of HDAC3 (arrow) using rabbit polyclonal HDAC3 Antibody (M1) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC3 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HDAC3 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
