Application
Western blot analysis of HELLS Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane).HELLS (arrow) was detected using the purified Pab.Application
HELLS Antibody (Center) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HELLS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HELLS Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HELLS Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HELLS; PASG; SMARCA6; Lymphoid-specific helicase; Proliferation-associated SNF2-like protein; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PASG SMARCA6 |
|---|---|
| Alternative Names | HELLS PASG SMARCA6 Lymphoid-specific helicase Proliferation-associated SNF2-like protein SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6 |
| Molecular Weight(MW) | 97074 Da |
| Function | Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis (By similarity) |
| Tissue Specificity | Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias |
| Cellular Localization | Nucleus (By similarity). Note=Closely associated with pericentric heterochromatin (By similarity) |
| Entrez Gene | 3070 |
|---|
Application
Western blot analysis of HELLS Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane).HELLS (arrow) was detected using the purified Pab.
Application
HELLS Antibody (Center) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HELLS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HELLS Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
