Application
HEPACAM2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the HEPACAM2 antibody detected the HEPACAM2 protein (arrow).| Product Name | HEPACAM2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HEPACAM2; MIKI; HEPACAM family member 2; Mitotic kinetics regulator |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MIKI |
|---|---|
| Alternative Names | HEPACAM2 MIKI HEPACAM family member 2 Mitotic kinetics regulator |
| Molecular Weight(MW) | 51407 Da |
| Function | Required during prometaphase for centrosome maturation. Following poly-ADP-ribosylation (PARsylation) by TNKS, translocates from the Golgi apparatus to mitotic centrosomes and plays a key role in the formation of robust microtubules for prompt movement of chromosomes: anchors AKAP9/CG-NAP, a scaffold protein of the gamma-tubulin ring complex and promotes centrosome maturation |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Golgi apparatus membrane; Single-pass type I membrane protein. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, centrosome. Midbody. Note=In interphase, localizes to the Golgi apparatus. Localizes to centrosomes and spindles during prophase, prometaphase, and metaphase of mitosis, and to midbodies at telophase. Translocation to mitotic centrosomes is the result of poly-ADP-ribosylation (PARsylation) |
| Entrez Gene | 253012 |
|---|---|
| Nucleotide Accession | NP_937794.1 |
Application
HEPACAM2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the HEPACAM2 antibody detected the HEPACAM2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
