Application
Western blot analysis on SKBR3 cell lysates using anti-Phospho-HER2 (pT1221/T1222) RabMAb (Cat. #AJ1340e), 1:5000 dilution. Cells were either (A) untreated (B) treated with Lambda phosphatase.| Product Name | HER2 Antibody Phospho (pT1221/T1222) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERBB2, HER2, NEU, NGL, Receptor tyrosine-protein kinase erbB-2, p185erbB2;C-erbB-2;NEU proto-oncogene;Tyrosine kinase-type cell surface receptor HER2;MLN 19 |
| Modification | p-T1221/T1222 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1047 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HER2 MLN19 NEU NGL |
|---|---|
| Alternative Names | ERBB2 HER2 NEU NGL Receptor tyrosine-protein kinase erbB-2 p185erbB2 C-erbB-2 NEU proto-oncogene Tyrosine kinase-type cell surface receptor HER2 MLN 19 |
| Molecular Weight(MW) | 137910 Da |
| Function | Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization |
| Tissue Specificity | Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1 Isoform 3: Cytoplasm. Nucleus. |
| Entrez Gene | 2064 |
|---|
Application
Western blot analysis on SKBR3 cell lysates using anti-Phospho-HER2 (pT1221/T1222) RabMAb (Cat. #AJ1340e), 1:5000 dilution. Cells were either (A) untreated (B) treated with Lambda phosphatase.| Application Notes | WB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
