Application
Western blot analysis of Syncytin(ERVWE1) (arrow) using rabbit polyclonal Syncytin(ERVWE1) Antibody (Center) (RB9254).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Syncytin(ERVWE1) gene (Lane 2) (Origene Technologies).| Product Name | HERV (ERVWE1) Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERVW-1; ERVWE1; HERV-W_7q21.2 provirus ancestral Env polyprotein; Endogenous retrovirus group W member 1; Env-W; Envelope polyprotein gPr73; Enverin; HERV-7q Envelope protein; HERV-W envelope protein; Syncytin; Syncytin-1; Surface protein; gp50; Transmembr |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ERVWE1 |
|---|---|
| Alternative Names | ERVW-1 ERVWE1 HERV-W_7q21.2 provirus ancestral Env polyprotein Endogenous retrovirus group W member 1 Env-W Envelope polyprotein gPr73 Enverin HERV-7q Envelope protein HERV-W envelope protein Syncytin Syncytin-1 Surface protein gp50 Transmembr |
| Molecular Weight(MW) | 59866 Da |
| Function | Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis The transmembrane protein (TM) acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of membranes (By similarity) |
| Tissue Specificity | Expressed at higher level in placental syncytiotrophoblast. Expressed at intermediate level in testis Seems also to be found at low level in adrenal tissue, bone marrow, breast, colon, kidney, ovary, prostate, skin, spleen, thymus, thyroid, brain and trachea. Both mRNA and protein levels are significantly increased in the brain of individuals with multiple sclerosis, particularly in astrocytes and microglia |
| Cellular Localization | Transmembrane protein: Cell membrane; Single-pass type I membrane protein (By similarity) HERV-W_7q21.2 provirus ancestral Env polyprotein: Virion (By similarity) |
| Entrez Gene | 30816 |
|---|
Application
Western blot analysis of Syncytin(ERVWE1) (arrow) using rabbit polyclonal Syncytin(ERVWE1) Antibody (Center) (RB9254).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Syncytin(ERVWE1) gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
