Application
HEYL Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HEYL antibody detected the HEYL protein (arrow).Application
Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term)(Cat#AP16997a).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.| Product Name | HEYL Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HEYL; BHLHB33; HRT3; Hairy/enhancer-of-split related with YRPW motif-like protein; Class B basic helix-loop-helix protein 33; Hairy-related transcription factor 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHB33 HRT3 |
|---|---|
| Alternative Names | HEYL BHLHB33 HRT3 Hairy/enhancer-of-split related with YRPW motif-like protein Class B basic helix-loop-helix protein 33 Hairy-related transcription factor 3 |
| Molecular Weight(MW) | 35087 Da |
| Function | Downstream effector of Notch signaling which may be required for cardiovascular development (By similarity) Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 |
| Tissue Specificity | This HEYL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human HEYL. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 26508 |
|---|---|
| Nucleotide Accession | NP_055386.1 |
Application
HEYL Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HEYL antibody detected the HEYL protein (arrow).
Application
Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term)(Cat#AP16997a).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
