Application
HHAT Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HHAT antibody detected the HHAT protein (arrow).Application
HHAT Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HHAT Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HHAT Antibody (N-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HHAT Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MART2, SKI1 |
|---|---|
| Alternative Names | HHAT MART2 SKI1 Protein-cysteine N-palmitoyltransferase HHAT Hedgehog acyltransferase Melanoma antigen recognized by T-cells 2 Skinny hedgehog protein 1 |
| Molecular Weight(MW) | 57313 Da |
| Function | Catalyzes N-terminal palmitoylation of SHH; which is required for SHH signaling (By similarity). May bind GTP |
| Tissue Specificity | Ubiquitously expressed in normal tissues and cancer cell lines |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity) |
| Entrez Gene | 55733 |
|---|---|
| Nucleotide Accession | NP_001116306.1 |
Application
HHAT Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HHAT antibody detected the HHAT protein (arrow).
Application
HHAT Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HHAT Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HHAT Antibody (N-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
