Application
HIBCH Antibody (Center) western blot analysis in WiDr cell line lysates (15ug/lane).This demonstrates the HIBCH antibody detected the HIBCH protein (arrow).Application
HIBCH Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIBCH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HIBCH Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HIBCH Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HIBCH 3-hydroxyisobutyryl-CoA hydrolase mitochondrial 3-hydroxyisobutyryl-coenzyme A hydrolase |
|---|---|
| Molecular Weight(MW) | 43482 Da |
| Function | Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism Also hydrolyzes 3-hydroxypropanoyl-CoA |
| Tissue Specificity | Highly expressed in liver and kidney, also detected in heart, muscle and brain (at protein level). Not detected in lung |
| Cellular Localization | Mitochondrion (By similarity). |
Application
HIBCH Antibody (Center) western blot analysis in WiDr cell line lysates (15ug/lane).This demonstrates the HIBCH antibody detected the HIBCH protein (arrow).
Application
HIBCH Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIBCH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HIBCH Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
