Application
HIP1 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HIP1 antibody detected the HIP1 protein (arrow).Application
HIP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | HIP1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HIP1 Huntingtin-interacting protein 1 Huntingtin-interacting protein I |
|---|---|
| Molecular Weight(MW) | 116221 Da |
| Function | Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR)-mediated transcription. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. May play a functional role in the cell filament networks. May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors |
| Tissue Specificity | Ubiquitously expressed with the highest level in brain. Expression is up-regulated in prostate and colon cancer |
| Cellular Localization | Cytoplasm. Nucleus. Endomembrane system. Cytoplasmic vesicle, clathrin-coated vesicle membrane Note=Shuttles between cytoplasm and nucleus. Nuclear translocation can be induced by AR |
| Entrez Gene | 3092 |
|---|---|
| Nucleotide Accession | NP_005329.3 |
Application
HIP1 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HIP1 antibody detected the HIP1 protein (arrow).
Application
HIP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
