Application
Western blot analysis of HIF1Alpha Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.Application
Western blot analysis of HIF1Alpha Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of HIF1Alpha Antibody (C-term)(Cat#AP7759b) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIF1Alpha antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohis| Product Name | HIF1Alpha Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHE78, MOP1, PASD8 |
|---|---|
| Alternative Names | HIF1A BHLHE78 MOP1 PASD8 Hypoxia-inducible factor 1-alpha ARNT-interacting protein Basic-helix-loop-helix-PAS protein MOP1 Class E basic helix-loop-helix protein 78 Member of PAS protein 1 PAS domain-containing protein 8 |
| Molecular Weight(MW) | 92670 Da |
| Function | Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia |
| Tissue Specificity | Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic in normoxia, nuclear translocation in response to hypoxia Colocalizes with SUMO1 in the nucleus, under hypoxia |
| Entrez Gene | 3091 |
|---|
Application
Western blot analysis of HIF1Alpha Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.
Application
Western blot analysis of HIF1Alpha Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of HIF1Alpha Antibody (C-term)(Cat#AP7759b) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIF1Alpha antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohis| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
