Application
HIF3A Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HIF3A antibody detected the HIF3A protein (arrow).Application
HIF3A antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIF3A antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | HIF3A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHE17, MOP7, PASD7 |
|---|---|
| Alternative Names | HIF3A BHLHE17 MOP7 PASD7 Hypoxia-inducible factor 3-alpha Basic-helix-loop-helix-PAS protein MOP7 Class E basic helix-loop-helix protein 17 HIF3-alpha-1 Inhibitory PAS domain protein Member of PAS protein 7 PAS domain-containing protein 7 |
| Molecular Weight(MW) | 72433 Da |
| Function | Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea (By similarity) |
| Tissue Specificity | Expressed in kidney. Expressed abundantly in lung epithelial cells. Expression is regulated in an oxygen- dependent manner |
| Cellular Localization | Nucleus. Cytoplasm. Note=In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes (By similarity) |
| Entrez Gene | 64344 |
|---|---|
| Nucleotide Accession | NP_690007.1 NP_690008.2 NP_071907.3 |
Application
HIF3A Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HIF3A antibody detected the HIF3A protein (arrow).
Application
HIF3A antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIF3A antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
