Application
Western blot analysis of HIPK1 Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in MCF-7 cell line lysate. HIPK1 Antibody (C-term) (arrow) was detected using the purified Pab (1:60/250 dilution).Application
HIPK1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIPK1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | HIPK1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0630, MYAK, NBAK2 |
|---|---|
| Alternative Names | HIPK1 KIAA0630 MYAK NBAK2 Homeodomain-interacting protein kinase 1 Nuclear body-associated kinase 2 |
| Molecular Weight(MW) | 130843 Da |
| Function | Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti- oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation |
| Tissue Specificity | Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. Isoform 2 is highly expressed in testis |
| Cellular Localization | Nucleus. Cytoplasm. Note=Predominantly nuclear. Translocates from nucleus to cytoplasm in response to stress stimuli via SENP1-mediated desumoylation |
| Entrez Gene | 204851 |
|---|---|
| Nucleotide Accession | NP_938009.1 NP_938010.1 |
Application
Western blot analysis of HIPK1 Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in MCF-7 cell line lysate. HIPK1 Antibody (C-term) (arrow) was detected using the purified Pab (1:60/250 dilution).
Application
HIPK1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIPK1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
