Application
Western blot analysis of HIST1H1B Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane).HIST1H1B (arrow) was detected using the purified Pab.Application
HIST1H1B Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HIST1H1B Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HIST1H1B; H1F5; Histone H1.5; Histone H1a |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H1F5 |
|---|---|
| Alternative Names | HIST1H1B H1F5 Histone H1.5 Histone H1a |
| Molecular Weight(MW) | 22580 Da |
| Function | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) |
| Tissue Specificity | Ubiquitous. Expressed in the majority of the cell lines tested and in testis |
| Cellular Localization | Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin |
| Entrez Gene | 3009 |
|---|
Application
Western blot analysis of HIST1H1B Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane).HIST1H1B (arrow) was detected using the purified Pab.
Application
HIST1H1B Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
