Application
HIST1H1D Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HIST1H1D antibody detected the HIST1H1D protein (arrow).Application
HIST1H1D Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIST1H1D Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | HIST1H1D Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H1F3 |
|---|---|
| Alternative Names | HIST1H1D H1F3 Histone H1.3 Histone H1c Histone H1s-2 |
| Molecular Weight(MW) | 22350 Da |
| Function | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) |
| Tissue Specificity | This HIST1H1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-164 amino acids from the Central region of human HIST1H1D. |
| Cellular Localization | Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in euchromatin. According to PubMed:10997781 is associated with inactive chromatin |
| Entrez Gene | 3007 |
|---|---|
| Nucleotide Accession | NP_005311.1 |
Application
HIST1H1D Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HIST1H1D antibody detected the HIST1H1D protein (arrow).
Application
HIST1H1D Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIST1H1D Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
