Application
HIST1H2AD Antibody (C-term) western blot analysis in 293,MDA-MB435,K562,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST1H2AD antibody detected the HIST1H2AD protein (arrow).Application
HIST1H2AD Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2AD Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HIST1H2AD Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HIST1H2AD Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H2AFG |
|---|---|
| Alternative Names | HIST1H2AD H2AFG Histone H2A type 1-D Histone H2A.3 Histone H2A/g |
| Molecular Weight(MW) | 14107 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | This HIST1H2AD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101~130 amino acids from the C-terminal region of human HIST1H2AD. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 3013 |
|---|
Application
HIST1H2AD Antibody (C-term) western blot analysis in 293,MDA-MB435,K562,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST1H2AD antibody detected the HIST1H2AD protein (arrow).
Application
HIST1H2AD Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2AD Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HIST1H2AD Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
