Application
HIST2H2BF Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
HIST2H2BF Antibody (C-term) western blot analysis in K562,A549 cell line lysates (35ug/lane).This demonstrates the HIST2H2BF antibody detected the HIST2H2BF protein (arrow).| Product Name | HIST2H2BF Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| FC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HIST2H2BF Histone H2B type 2-F |
|---|---|
| Molecular Weight(MW) | 13920 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | This HIST2H2BF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-125 amino acids from the C-terminal region of human HIST2H2BF. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 440689 |
|---|---|
| Nucleotide Accession | NP_001019770.1 |
Application
HIST2H2BF Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
HIST2H2BF Antibody (C-term) western blot analysis in K562,A549 cell line lysates (35ug/lane).This demonstrates the HIST2H2BF antibody detected the HIST2H2BF protein (arrow).| Application Notes | FC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
