Application
A. Western blot analysis on K562 cell lysate using anti-Histone Deacetylase 2 (C-term) RabMAb (Cat. #AJ1347a), 1:2,000Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Histone Deacetylase 2 (C-term) RabMAb (Cat. #AJ1347a).Application
C. Immunofluorescent analysis of HeLa cells using anti-Histone Deacetylase 2 (C-term) RabMAb (Cat. #AJ1347a).| Product Name | Histone Deacetylase 2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y461 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HDAC2 Histone deacetylase 2 |
|---|---|
| Molecular Weight(MW) | 55364 Da |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development |
| Tissue Specificity | Widely expressed; lower levels in brain and lung |
| Cellular Localization | Nucleus. |
| Entrez Gene | 3066 |
|---|
Application
A. Western blot analysis on K562 cell lysate using anti-Histone Deacetylase 2 (C-term) RabMAb (Cat. #AJ1347a), 1:2,000
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Histone Deacetylase 2 (C-term) RabMAb (Cat. #AJ1347a).
Application
C. Immunofluorescent analysis of HeLa cells using anti-Histone Deacetylase 2 (C-term) RabMAb (Cat. #AJ1347a).| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
