Application
A. Western blot analysis on K562 cell lysate using anti-Histone Deacetylase 3 (C-term) RabMAb (Cat. #AJ1348a), 1:10,000Application
B. Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-Histone deacetylase 3 (C-term) RabMAb (Cat. #AJ1348a).Application
C. Immunofluorescent staining of HeLa cells using anti-Histone deacetylase 3 (C-term) RabMAb (Cat. #AJ1348a).| Product Name | Histone Deacetylase 3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y415 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HDAC3 Histone deacetylase 3 RPD3-2 SMAP45 |
|---|---|
| Molecular Weight(MW) | 48848 Da |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (By similarity) |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 8841 |
|---|
Application
A. Western blot analysis on K562 cell lysate using anti-Histone Deacetylase 3 (C-term) RabMAb (Cat. #AJ1348a), 1:10,000
Application
B. Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-Histone deacetylase 3 (C-term) RabMAb (Cat. #AJ1348a).
Application
C. Immunofluorescent staining of HeLa cells using anti-Histone deacetylase 3 (C-term) RabMAb (Cat. #AJ1348a).| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
