Application
A. Western blot analysis on C6 cell lysates using anti- Histone H3TB Acetyl K14 RabMAb (Cat. #AJ1353b), 1:2000 dilution. Cells were either (A) untreated (B) treated with TSA.Application
B. Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of uterus using anti Histone H3TBRabMAb(Cat. #AJ1353b).Application
C. Immunofluorescent staining of HeLa cells using anti-Histone H3TB RabMAb (Cat. #AJ1353b).| Product Name | Histone H3 Antibody Acetyl (K14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | Acetyl K14 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP964Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H3FA |
|---|---|
| Alternative Names | HIST1H3J H3FJ Histone H3.1 H3/a H3/b H3/c H3/d H3/f H3/h H3/i H3/j H3/k H3/l |
| Molecular Weight(MW) | 15404 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | A synthetic acetyl-specific peptide corresponding to residues around Lysine 14 was used as an immunogen. This antibody recognizes Histone H3 acetylated on K14. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 8968 |
|---|
Application
A. Western blot analysis on C6 cell lysates using anti- Histone H3TB Acetyl K14 RabMAb (Cat. #AJ1353b), 1:2000 dilution. Cells were either (A) untreated (B) treated with TSA.
Application
B. Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of uterus using anti Histone H3TBRabMAb(Cat. #AJ1353b).
Application
C. Immunofluorescent staining of HeLa cells using anti-Histone H3TB RabMAb (Cat. #AJ1353b).| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
